Domestication of a wild useful plant:
In vitro cultivation of the Wild Indigo (Baptisia tinctoria)

The Fabacea Baptisia tinctoria, known as Wild Indigo, is a widely branched perennial, which is found in Eastern North America. The use of B. tinctoria roots and rhizomes in traditional American medicine is well known, today the drug is an ingredient in immunomodulating pharmaceuticals, e.g. ESBERITOX® (Schaper & Brümmer). The drug is still collected from its natural habitat in the US with the risk of diverse quality and possible falsification with related species. As B. tinctoria reproduces slowly, the natural ressources would soon be endangered. It was therefore prudent to establish an agricultural method for obtaining high quality material.

Traditional cultivation from seeds proved impractical due to their hardseededness and low germination rate. Therefore, in vitro propagation of plants had to be established at the Institute for Applied Botany of the University of Hamburg during 1991-1994.
Seeds were collected from plants with high quality properties among the wild endemic plant population of Ohio/USA. They were scarified, surface sterilized and brought to germination within a week. From the plantlets subsequently obtained meristem cultures were prepared using stem cuts. Using this approach, mass propagation was established at an industrial scale. It is possible and actually done to produce large quantities of plantlets from a collection of elite-clones. The adaptation of the plantlets from in vitro to greenhouse conditions proved difficult. However, it was possible to improve the survival rate considerably over the last few years. With respect to that survival rate symbiotic micro-organisms were proved to be advantegous. After these initial studies the technology were outsourced to a spin off, the Institute for Plant Cultivation.

There, plantlets are now transferred to the greenhouse in March were they are held at high humidity and appropriate temperature. One of the selection parameters used at this stage of acclimatization is mildew resistance. The adapted plantlets are then transferred to the fields in May and cultivated for three years. Currently about 8-10 hectares are grown with Baptisia tinctoria according to GAP rules (´Good Agricultural Practice for medicinal and aromatic Plant Production´). Because of its very slow growth rate B. tinctoria is easily suppressed by weed. During the growth phase in summer manual weed control is obligatory monthly. After three-year-cultivation the roots are harvested. B. tinctoria produces roots as long as 70 cm. For the harvest a vibrating plough usually used in forest nurseries loosens the soil to a depth of 60 cm. The plants can be picked out of the soil manually, the roots are not injured or torn. After harvest roots have to be dried immediately. For this reason a 10 m² drying facility was built in which the roots are dried in a constant warm air at 35 °C. For transport and storage purpose the roots need to be cut in 5 cm pieces.

Since the in vitro culture, the in vitro / ex vitro adaptation procedure and the field cultivation have now been established, the main aspect of ongoing work is the selection and propagation of high performance clones leading to large yields of high quality drug.

With respect to strategic management of product chains of agricultural products the exchange points between segments of a product chain offer the possibility to document and to evaluate the quality and reliability of information transfer by independent institutions. Here, possibly we will find a basis for recommendations to stakeholders which direction they should choose to increase the production quality and to enhance the sustainability of the related product chain.

Expertness

The author participated  in the interdisziplinary project by introducing Ph.-D. students and developing methods for micro-organism use during the weaning phase of plant cultivation.

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